Serodiagnosis of Aspergillosis

Characterization of AFMP1: a novel target for serodiagnosis of aspergillosis.

We cloned the AFMP1 gene, which encodes the first antigenic cell wall galactomannoprotein in Aspergillus fumigatus. AFMP1 codes for a protein, Afmp1p, of 284 amino acid residues, with a few sequence features that are present in Mp1p, the antigenic cell wall mannoprotein in Penicillium marneffei that we described previously, as well as several other cell wall proteins of Saccharomyces cerevisiae...

Development of an immunochromatographic lateral-flow device for rapid serodiagnosis of invasive aspergillosis.

Aspergillus fumigatus is a cosmopolitan saprotrophic fungus that is second only to Candida species as a cause of invasive fungal infections in immunocompromised humans. Current immunodiagnostic tests for invasive aspergillosis (IA) are based on the detection of circulating galactomannan (GM) in a patient's serum by using a rat monoclonal antibody (MAb), EB-A2, that binds to tetra (1-->5)-beta-D...

Detection of antibodies specific to an antigenic cell wall galactomannoprotein for serodiagnosis of Aspergillus fumigatus aspergillosis.

Aspergilloma and invasive aspergillosis are important opportunistic infections caused by Aspergillus species, among which Aspergillus fumigatus is the most common species associated with human disease. We developed an enzyme-linked immunosorbent assay (ELISA)-based antibody assay with Afmp1p, a purified recombinant antigenic cell wall galactomannoprotein of A. fumigatus. Evaluation of the test ...

Serodiagnosis of tuberculosis.

In the developing countries, which have the majority of the world's population, tuberculosis is one of the most important infectious diseases, causing illness and death on a very large scale. In these counlries there ha<; been little or no decline of this disease during recent years and improved conlrol programmes and case identification are therefore imperative. In the developed countries tube...

Pulmonary Aspergillosis in Ostrich